Phospho-DOK2 (Tyr351) Polyclonal Antibody

• Background:

  DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

• Synonyms:

  DokR; Frip; Docking protein 2; Dok-related protein; Dok-R; Downstream of tyrosine kinase 2; IL-four receptor-interacting protein; p56 (dok-2) ; Dok2

• Gene ID:

  13449

• Swiss Prot:

  O70469

• Modification Site:

  Tyr351

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Target:

  Phospho-DOK2 (Tyr351)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic phosphopeptide derived from mouse p56Dok2 around the phosphorylation site of (Tyr351)

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Specificity:

  This phosphorylation site is found at Tyr345 in Human. Due to the highly conserved nature of this sequence with DOK1, this antibody may react with DOK1 when it is phosphorylated at Tyr362 in Human and Tyr361 in Mouse and Rat.

• Gene ID URL:

  13449

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken