Rabbit anti Human Caspase-3

• Background:

  Caspase-3 along with caspase 7 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms. Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when cocultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation. For both wild-type and caspase-1 (-/-) apoptotic hepatocytes, typical apoptotic features such as cytoplasmic blebbing and nuclear fragmentation are seen within 6 hr, but neither event was observed for caspase-3(-/-) hepatocytes. In thymocytes apoptotic caspase-3 (-/-) thymocytes exhibit similar abnormal morphological changes and delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gelsolin, fodrin, laminB, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3.

• Synonyms:

  CPP32, Rabbit Anti caspase 3

• UniProt:

  P42574

• Host:

  Rabbit

• Species Reactivity:

  Human

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Field of Research:

  Apoptosis

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Applications: western blot, use at 2-10 µg/mL

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline, pH 7,3, with 1,0 mg/mL BSA and 0,05% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1] Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. Slee EA; Harte MT; Kluck RM; Wolf BB; Casiano CA; Newmeyer DD; Wang HG; Reed JC; Nicholson DW; Alnemri ES; Green DR; Martin SJ. J Cell Biol, 144(2):281-92 1999 Jan 25 2] Redox regulation of caspase-3(-like) protease activity: regulatory roles of thioredoxin and cytochrome c. Ueda S; Nakamura H; Masutani H; Sasada T; Yonehara S; Takabayashi A; Yamaoka Y; Yodoi J. J Immunol, 161(12):6689-95 1998 Dec 15 3] Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells. Samali A; Cai J; Zhivotovsky B; Jones DP; Orrenius S. EMBO J, 18(8):2040-8 1999 Apr 15

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4