E2F3 Polyclonal Antibody, RBITC Conjugated

• Background :

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].

• Synonyms :

  DKFZp686C18211; E2F 3; E2F transcription factor 3; KIAA0075; MGC104598; Transcription factor E2F 3; E2F3_HUMAN; Transcription factor E2F3; E2F-3.

• Gene ID :

  O00716

• Swiss Prot :

  1871

• Cellular Locus :

  Nucleus

• Host :

  Rabbit

• Cross Reactivity :

  Human, Mouse, Rat

• Immunogen :

  361-460/465

• Target :

  E2F3

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  RBITC

• Source :

  KLH conjugated synthetic peptide derived from human E2F3

• Applications :

  WB, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Excitation Emission :

  570nm/595nm

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Gene ID URL :

  O00716

• Predicted Cross Reactivity :

  Cow, Sheep, Horse, Rabbit