CD33 Polyclonal Antibody, APC Conjugated

• Background:

  Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro) .

• Synonyms:

  P67; SIGLEC3; SIGLEC-3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; gp67; CD33

• Gene ID:

  945

• Swiss Prot:

  P20138

• Cellular Locus:

  Cell membrane

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Immunogen:

  261-364/364

• Target:

  CD33

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  APC

• Source:

  KLH conjugated synthetic peptide derived from human CD33

• Applications:

  WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Excitation Emission:

  650nm/660nm

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Gene ID URL:

  945