Calsequestrin Polyclonal Antibody, PE Conjugated

• Background:

  The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

• Synonyms:

  CASQ; Calsequestrin 1; Calsequestrin 1 fast twitch skeletal muscle; Calsequestrin skeletal muscle isoform; CASQ 1; CASQ1; PDIB1; Skeletal muscle calsequestrin 1; Calmitine.

• Gene ID:

  844

• Swiss Prot:

  P31415

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Immunogen:

  1-100/396

• Target:

  Calsequestrin

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  PE

• Source:

  KLH conjugated synthetic peptide derived from human Calsequestrin

• Applications:

  WB, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Excitation Emission:

  496,564nm/578nm

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Gene ID URL:

  844

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit