HHAT Polyclonal Antibody

• Background:

  HHAT is a 493 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and belongs to the membrane-bound acyltransferase family. Expressed ubiquitously, HHAT functions to catalyze the N-terminal palmitoylation of SSH (slingshot homolog), an event that is required for SHH signaling pathways. HHAT is expressed in cancer cell lines, suggesting a role for HHAT in tumorigenesis. The gene encoding HHAT maps to human chromosome 1 and is expressed as four alternatively spliced isoforms. Chromosome 1 is the largest human chromosome, spanning about 260 million base pairs and making up 8% of the human genome. Several disorders, including Stickler syndrome, Parkinsons Disease, Gaucher disease, malignant melanoma and Usher syndrome, are caused by defects in genes that localize to chromosome 1.

• Synonyms:

  2810432O22Rik; AC115917.1; AI462858; FLJ10724; FLJ34867; Hedgehog acyltransferase; Hhat; HHAT_HUMAN; MART 2; MART-2; MART2; Melanoma antigen recognized by T cells 2; Melanoma antigen recognized by T-cells 2; MGC11697; OTTMUSP00000033669; Protein cysteine N palmitoyltransferase HHAT; Protein-cysteine N-palmitoyltransferase HHAT; RGD1311746; SKI1; Skinny hedgehog protein 1; Skinny hedgehog protein; Skn.

• Gene ID:

  55733

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Immunogen:

  101-200/493

• Target:

  HHAT

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human HHAT

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  55733

• Predicted Cross Reactivity:

  Human, Mouse, Rat