STAP2 Polyclonal Antibody

• Background :

  Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues (1,2) . Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states (1,2) . STAP-2 is a protein that contains a pleckstrin homology (PH) domain and an SH2 domain, and associates with BRK (3) . BRK (breast tumor kinase, Sik) is a 451 amino acid, nonreceptor protein-tyrosine kinase that is overexpressed in breast tumors and metastatic melanoma cell lines (4) . Similar to the Src family of intracellular kinses, BRK is comprised of an SH3 domain, an SH2 domain, and a catalytic domain (5) . STAP-2 is susceptiple to tyrosine phosphorylation and may be invovled in tyrosine kinase-mediated signaling cascades, whose aberrant function may lead to metastis (3) .

• Synonyms :

  BKS; Breast tumor kinase substrate; BRK substrate; Signal transducing adaptor protein 2; Signal-transducing adaptor protein 2; Ssignal transducing adaptor family member 2; STAP 2; STAP-2; STAP2; STAP2_HUMAN.

• Gene ID :

  55620

• Swiss Prot :

  Q9UGK3

• Cellular Locus :

  Cytoplasm

• Host :

  Rabbit

• Immunogen :

  151-250/403

• Target :

  STAP2

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human STAP2

• Applications :

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL :

  55620

• Predicted Cross Reactivity :

  Human