GPR40 Polyclonal Antibody

• Background:

  G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1) . May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity) . Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G (q) and G (i) -mediated pathway.

• Synonyms:

  FFA1R; GPR40; GPCR40; Free fatty acid receptor 1; G-protein coupled receptor 40; FFAR1

• Gene ID:

  2864

• Swiss Prot:

  O14842

• Cellular Locus:

  Cell membrane

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Immunogen:

  121-220/300

• Target:

  GPR40

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human GPR40

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  2864

• Predicted Cross Reactivity:

  Mouse, Rat, Dog, Pig