GCAP2 Polyclonal Antibody

• Background:

  The intracellular stimulation of guanylate cyclase (GC) by calcium, a key event in the recovery of the dark state of rod photoreceptors after exposure to light, is mediated by guanylate cyclase-activating protein (GCAP1) . GCAPs are calcium-binding proteins belonging to the calmodulin superfamily. GCAP1 is a calcium-binding protein that stimulates synthesis of cGMP in photoreceptors. GCAP1 is present in rod and cone photoreceptor outer segments where phototransduction occurs. In contrast to other calcium-binding proteins from the calmodulin superfamily, the calcium-free form of GCAP1 stimulates the effector enzyme. By molecular cloning of human and mouse GCAP cDNA, the known mammalian GCAPs are found to be more than 90% similar, consisting of 201 to 205 amino acids and containing three identically conserved calcium-binding sites. A related protein, GCAP2, is detectable only in the retina and results from a gene duplication event. The genes which encode GCAP1 and GCAP2 map to human chromosome 6p21.1.

• Synonyms:

  GCAP 2; Guanylate Cyclase Activating Protein Photoreceptor 2; Guanylate Cyclase Activator 1B; Guanylin 2; Guanylyl cyclase-activating protein 2; GUC1B_HUMAN; GUCA 2; GUCA1B.

• Gene ID:

  2979

• Cellular Locus:

  Cell membrane

• Host:

  Rabbit

• Immunogen:

  21-120/200

• Target:

  GCAP2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human GCAP2

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  2979

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Cow, Sheep, Horse, Rabbit