EYA4 Polyclonal Antibody

• Background:

  A gene of chromosome 6q23 encodes the 640 amino acid protein, EYA4 (eyes absent) (1) . EYA is one of four members of the eyes absent family (1) . A 271 amino acid domain at the carboxyl terminal is highly conserved amongst the members of the eyes absent family (1) . EYA4 is expressed in the craniofacial mesenchyme, the dermamyotome, and the limb (1) . The conserved region in other EYA proteins interacts with SIX, DACH, and G-proteins, which regulate transcription in early embryonic development (1,2,3,4) . SIX translocates EYA1-3 to the nucleus, and G-proteins can stop this interaction (3,4) . Premature stop codon mutations in EYA4 cause postlingual, progressive autosomal dominant hearing loss in humans (2) . This shows that EYA4 is also vital to the mature organ of Corti (2) . EYA4 may cause oculo-dento-digital syndrome, based on its expression pattern and map postion (1) .

• Synonyms:

  CMD1J; Deafness, autosomal dominant 10; DFNA 10; DFNA10; dJ78N10.1 eyes absent Drosophila homolog 4; dJ78N10.1 eyes absent; EYA 4; eya4; EYA4_HUMAN; Eyes absent 4; Eyes absent homolog 4 Drosophila; Eyes absent homolog 4; HGNC:3522; OTTHUMP00000040267.

• Gene ID:

  2070

• Cellular Locus:

  Cytoplasm, Nucleus

• Host:

  Rabbit

• Immunogen:

  301-400/639

• Target:

  EYA4

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human EYA4

• Applications:

  ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  2070

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse