CD59 Polyclonal Antibody

• Background:

  The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI) -linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-?production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.

• Synonyms:

  Ly 6c; Ly6c protein; Ly-6C; Ly6c1; Ly6c2; Lymphocyte antigen 6 complex; Lymphocyte antigen 6 complex locus C; Lymphocyte antigen 6C; Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen Ly 6C; CD59_HUMAN.

• Cellular Locus:

  Cell membrane

• Host:

  Rabbit

• Immunogen:

  26-102/128

• Target:

  CD59

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human Ly6c

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Predicted Cross Reactivity:

  Human