PPT2 Polyclonal Antibody

• Background:

  PPT2 (palmitoyl-protein thioesterase 2), also known as G14, is a 302 amino acid glycosylated protein that localizes to the lysosome and belongs to the palmitoyl-protein thioesterase family. Expressed throughout the body with highest levels in skeletal muscle, PPT2 functions to remove thioester-linked fatty acyl groups from a variety of substrates, including S-palmitoyl-CoA, thereby playing an important role in lipid metabolism. PPT2 operates at an optimal pH of 7 and exhibits the highest activity for the acyl groups on myristic and palmitic acids, with lower levels of activity toward other short- and long-chain acyl substrates. PPT2 exists as two isoforms, one of which is expressed at low levels and is catalytically inactive.

• Synonyms:

  Lysosomal thioesterase PPT2; Palmitoyl protein hydrolase 2; Palmitoyl protein thioesterase 2; PPT 2; PPT-2; Ppt2; PPT2_HUMAN; S thioesterase G14; S-thioesterase G14.

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Immunogen:

  221-302/302

• Target:

  PPT2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human PPT2

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Cow, Pig, Horse