Slit1 Polyclonal Antibody

• Background:

  Secreted leucine-rich repeat-containing proteins 1-3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural development by acting as high-affinity signaling ligands for the repulsive guidance receptor, Roundabout (Robo) (1, 2) . Within the developing central nervous system (CNS) of different vertebrate systems, Slit proteins are expressed in equivalent regions, suggesting a conserved function among vertebrate homologs (3,4) . Slit is expressed in the midline of the central nervous system in both vertebrates and invertebrates, where it functions as a regulatory factor of mesodermal cell movement during gastrulation (5) . Slit2 is a short range inhibitory guidance cue for retinal ganglion cell (RGC) axons that may mediate spatial progression of RGCs (6,7) .

• Synonyms:

  MEGF 4; MEGF4; Multiple EGF like domains protein 4; Multiple epidermal growth factor like domains 4; Multiple epidermal growth factor like domains protein 4; SLIL 1; SLIL1; Slit 1; SLIT 3; Slit homolog 1; Slit homolog 1 protein; Slit-1; Slit1; SLIT3; KIAA0813; SLIT1_HUMAN.

• Cellular Locus:

  Secreted

• Host:

  Rabbit

• Immunogen:

  455-500/1534

• Target:

  Slit1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human Slit1/MEGF4

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit