MID2 Polyclonal Antibody

• Background:

  Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4) . Dimerization is mediated by the tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.

• Synonyms:

  FXY2; MID2; Midin-2; Midline 2; Midline defect 2; Midline-2; Probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; Ring finger protein protein 60; RNF60; TRIM1; TRIM1_HUMAN; Tripartite mot protein 1; Tripartite mot-containing protein 1; Tripartite mot-containing protein 1; Midline defect 2; FLJ37715; FLJ41813.

• Gene ID:

  26040

• Host:

  Rabbit

• Immunogen:

  251-350/735

• Target:

  MID2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human RNF60/Midline-2

• Applications:

  ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  26040

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Dog, Sheep, Pig, Horse, Chicken