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Phospho-MCM2 (Ser53) Polyclonal Antibody

CAT: 0498-bs-10608RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-10608RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Synonyms
MCM4 (phospho S53) ; MCM2 (phospho-Ser53) ; MCM2 (phospho Ser53) ; MCM2 (phospho S54) ; p-MCM2 (Ser53) ; p-MCM2 (S53) ; rat MCM4 (phospho S54) ; BM28; CCNL 1; CCNL1; CDC like 1; CDC like-1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; cyclin like-1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) ; MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MCM2_MOUSE; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin) ; Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050.
Gene ID
4171
Swiss Prot
P49736
Modification Site
Ser53
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-MCM2 (Ser53)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated Synthesised phosphopeptide derived from human MCM2 around the phosphorylation site of Ser53
Applications
ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
4171
Predicted Cross Reactivity
Mouse, Rat, Dog, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish

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