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MYLPF Polyclonal Antibody

CAT: 0498-bs-5159R-TRSize: 20 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-5159R-TRSize:20 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
Synonyms
MRLC2; MYL11; HUMMLC2B; Myosin regulatory light chain 2, skeletal muscle isoform; Fast skeletal myosin light chain 2; MLC2B; MYLPF
Gene ID
29895
Swiss Prot
Q96A32
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
101-170/170
Target
MYLPF
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human MRLC2
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
This antibody may recognize myosin light chain 5, myosin light chain 7, or myosin regulatory light chain 10
Prediction Reactivity
Dog, Cow, Sheep, Pig, Horse, Rabbit
Gene ID URL
29895
Isotype
IgG

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