FLASH Polyclonal Antibody

• Background:

  FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity.Studies of FLASH protein suggested that this protein may be a component of the death inducing signaling complex that includes Fas receptor, Fas binding adapter FADD, and caspase 8, and plays a regulatory role in Fas mediated apoptosis.

• Synonyms:

  CASP 8 associated protein 2; CASP8 associated protein 2; CASP8AP2; Caspase 8 associated protein 2; CED 4; CED4; FLASH homolog; FLASH homolog RIP 25; FLASH homolog RIP25; FLICE associated huge; FLICE associated huge protein; FLICE-associated huge protein; RIP 25; RIP25; AA387232; D4Ertd659e; mKIAA1315; C8AP2_HUMAN; CASP8-associated protein 2; FLICE-associated huge protein; FLJ11208; KIAA1315; OTTMUSP00000005125; RP23-69G24.1.

• Gene ID:

  9994

• Cellular Locus:

  Cytoplasm, Nucleus

• Host:

  Rabbit

• Cross Reactivity:

  Mouse

• Immunogen:

  1861-1982/1982

• Target:

  FLASH

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human FLASH/CASP8AP2

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1ug/ul

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity:

  Human, Rat, Dog, Cow, Pig, Horse

• Gene ID URL:

  9994

• Isotype:

  IgG