LC3B Polyclonal Antibody

• Background:

  A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.

• Synonyms:

  MAP LC3 beta; LC3; Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modier LC3 A; Autophagy related ubiquitin like modier LC3 B; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1ALC3; MAP1BLC3; MAP1LC3A; MAP1LC3B; Microtubule associated protein 1 light chain 3 alpha; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3; Microtubule associated proteins 1A/1B light chain 3A; Microtubule associated proteins 1A/1B light chain 3B.microtubule-associated proteins 1A/1B light chain 3 beta 2

• Gene ID:

  81631, 84557

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Mouse, Rat, Rabbit

• Immunogen:

  21-121/121

• Target:

  LC3B

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human MAP1A/MAP1B LC3 B

• Applications:

  IHC-P, IHC-F, IF

• Purification:

  Purified by Protein A.

• Concentration:

  1ug/ul

• Dilution:

  IHC-P (1:100-500), IHC-F (1:100-500), IF (1:100-500)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity:

  Human, Dog, Cow, Pig, Horse, Chicken, Zebrafish

• Gene ID URL:

  81631, 84557

• Isotype:

  IgG