P4HB Polyclonal Antibody

• Background :

  This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity) . May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307) .

• Synonyms :

  Protein Disulfide lsomerase; Cellular thyroid hormone-binding protein; PDI; Prolyl 4-hydroxylase subunit beta; p55; P4HB; ERBA2L; P4HB; ERBA2L; PDIA1; PO4DB; PDIA1_HUMAN.

• Gene ID :

  5034

• Swiss Prot :

  P07237

• Cellular Locus :

  Cytoplasm

• Host :

  Rabbit

• Cross Reactivity :

  Human, Mouse

• Immunogen :

  21-150/508

• Target :

  P4HB

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human PDI

• Applications :

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1ug/ul

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity :

  Rat, Cow, Horse, Chicken, Rabbit

• Gene ID URL :

  5034

• Isotype :

  IgG