APPBP1 Polyclonal Antibody

• Background :

  Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

• Synonyms :

  HPP1; ula-1; APPBP1; A-116A10.1; NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1; NAE1

• Gene ID :

  8883

• Swiss Prot :

  Q13564

• Cellular Locus :

  Cell membrane

• Host :

  Rabbit

• Cross Reactivity :

  Human, Mouse, Rat

• Immunogen :

  401-500/534

• Target :

  APPBP1

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human APPBP1

• Applications :

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1ug/ul

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity :

  Dog, Pig, Horse, Rabbit

• Gene ID URL :

  8883

• Isotype :

  IgG