MGMT Polyclonal Antibody

• Background :

  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

• Synonyms :

  Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

• Gene ID :

  4255

• Swiss Prot :

  P16455

• Cellular Locus :

  Nucleus

• Host :

  Rabbit

• Cross Reactivity :

  Rat

• Immunogen :

  101-207/207

• Target :

  MGMT

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human MGMT

• Applications :

  WB, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1ug/ul

• Dilution :

  WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity :

  Human, Mouse

• Gene ID URL :

  4255

• Isotype :

  IgG