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Rabbit Anti-Avidin

CAT: 0498-bs-0312RSize: 1 mgDry Ice: NoHazardous: No
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CAT#:0498-bs-0312RSize:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05 percent of total protein (approximately 1.8 mg per egg) . The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. In its tetrameric form, avidin is estimated to be between 66 to 69 kDa in size.Ten percent of the molecular weight is attributed to carbohydrate content composed of four to five mannose and three N acetylglucosamine residues. The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition.
Host
Rabbit
Cross Reactivity
Avidin
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Applications
WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Affinity purified by Protein A
Concentration
10mg/ml
Dilution
WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Lyophilized
Storage Conditions
Store at -20°C for 12 months.

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