NANOG Monoclonal Antibody

• Background:

  Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation.

• Synonyms:

  NANOG_MOUSE; Embryonic stem cell specific homeobox protein (Nanog) ; ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4.

• Gene ID:

  79923

• Swiss Prot:

  Q80Z64

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Target:

  NANOG

• Clonality:

  Monoclonal

• Clone:

  N3F1

• Conjugation:

  Unconjugated

• Source:

  Recombinant human NANOG.

• Applications:

  WB, IHC-P, IHC-F, IF

• Concentration:

  0.5µg/µl

• Dilution:

  WB (WB=1:500-2000), IHC-P (IHC-P=1:400-800), IHC-F (IHC-F=1:400-800), IF (IF=1:100-500)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  79923

• Predicted Cross Reactivity:

  Human