Histone H3 (tri methyl K4)

• Background:

  Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Synonyms:

  Histone H3 (tri methyl Lys4) ; H3K4me3; H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD

• Gene ID:

  8350

• Swiss Prot:

  P68431

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Cross Reactivity:

  Human

• Target:

  H3C1

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  3D10

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K4

• Applications:

  WB, IHC-P, IHC-F, IF

• Purification:

  Purified by Protein A.

• Concentration:

  1mg/ml

• Dilution:

  WB (WB=1:500-1000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:400-800), IF (IF=1:100-500)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  8350

• Predicted Cross Reactivity:

  Mouse, Rat