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ESD Antibody / Esterase D

CAT: 0800-FY12168Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY12168Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P10768
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human ESD recombinant protein (Position: M1-A282) was used as the immunogen for the ESD antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ICC/IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This ESD antibody is available for research use only.
Storage Conditions
After reconstitution, the ESD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the ESD antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Immunofluorescent staining of ESD using anti-ESD antibody (green) and anti-Beta Tubulin antibody (red) . ESD was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-ESD antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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