CD21 Antibody / Complement receptor type 2

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

• UniProt:

  P20023

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  E.coli-derived human CD21/CR2 recombinant protein (Position: D76-N817) was used as the immunogen for the CD21 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This CD21 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD21 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the CD21 antibody should be determined by the researcher.

• Image Legend:

  Western blot analysis of CD21/CR2 using anti-CD21 antibody. Lane 1: mouse Raw264.7 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD21 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for CD21/CR2 is at 110-120 kDa (multiple isoforms), but it may be observed at higher molecular weights due to glycosylation.