SIPA1 Antibody / Signal-induced proliferation-associated 1

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q96FS4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E.coli-derived human SIPA1 recombinant protein (Position: D21-L1034) was used as the immunogen for the SIPA1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ICC/IF, FACS, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This SIPA1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the SIPA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the SIPA1 antibody should be determined by the researcher.

• Location:

  Nuclear, Perinuclear

• Image Legend:

  Immunofluorescent staining of SIPA1 using anti-SIPA1 antibody (green) and anti-Beta Tubulin antibody (red) . SIPA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SIPA1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.