DKC1 Antibody / Dyskerin

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  O60832

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E.coli-derived human DKC1 recombinant protein (Position: R19-R447) was used as the immunogen for the DKC1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC, ICC/IF, FACS, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This DKC1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the DKC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the DKC1 antibody should be determined by the researcher.

• Location:

  Nucleus, Nucleolus, Cytoplasm

• Image Legend:

  Immunofluorescent staining of DKC1 using anti-DKC1 antibody (green) and anti-Beta Tubulin antibody (red) . DKC1 was detected in an immunocytochemical section of human U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-DKC1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight?594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.