PECR Antibody / Peroxisomal trans-2-enoyl-CoA reductase

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

• UniProt:

  Q9BY49

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence in the middle region of human PECR was used as the immunogen for the PECR antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC, IF

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This PECR antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PECR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the PECR antibody should be determined by the researcher.

• Location:

  Cytoplasm (Peroxisome)

• Image Legend:

  Immunofluorescent staining of PECR using anti-PECR antibody (red) . PECR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-PECR antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.