Products for Research Use Only

BCL9L Antibody

CAT: 0800-RQ4979Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4979Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903) . There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.
Specifications
Western blot: 0.1-0.5 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)
UniProt
Q86UU0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids E397-A666 from the human protein were used as the immunogen for the BCL9L antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Limitations
This BCL9L antibody is available for research use only.
Storage Conditions
Store the BCL9L antibody at -20°C.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the BCL9L antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human esophagus squama cancer with BCL9L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.