EZH2 Antibody

• Description:

  Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme encoded by EZH2gene. It is mapped to 7q36.1. This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2ug/500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q15910

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids E22-T345 from the human protein were used as the immunogen for the EZH2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, IP, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This EZH2 antibody is available for research use only.

• Storage Conditions:

  Store the EZH2 antibody at -20°C.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the EZH2 antibody should be determined by the researcher.

• Image Legend:

  Immunoprecipitation of EZH2 protein from 500ug of human MCF7 whole cell lysate with 2ug of EZH2 antibody.