Hephaestin Antibody / HEPH

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q9BQS7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A human recombinant partial protein (amino acids E348-Q1157) was used as the immunogen for the Hephaestin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This Hephaestin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Hephaestin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Hephaestin antibody should be determined by the researcher.

• Image Legend:

  IHC staining of FFPE human small intestine tissue with Hephaestin antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.