MyoD1 Antibody

• Description:

  Recognizes a phosphor-protein of 45kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

• Specifications:

  Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt:

  P15172

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat.

• Immunogen:

  Recombinant mouse MyoD1 protein was used as the immunogen for this MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  5.8A

• Applications:

  FACS, IF, IHC

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Limitations:

  This MyoD1 antibody is available for research use only.

• Storage Conditions:

  Store the MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Location:

  Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

• Image Legend:

  IHC staining of FFPE human Rhabdomyosarcoma with MyoD1 antibody (clone 5.8A) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.