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MyoD1 Antibody

CAT: 0800-V3168-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3168-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
Specifications
Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
UniProt
P15172
Host
Mouse
Reactivity
Human, Mouse, Rat.
Immunogen
Recombinant mouse MyoD1 protein was used as the immunogen for this MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
5.8A
Applications
FACS, IF, IHC
Purity
Protein G affinity chromatography
Format
Purified
Limitations
This MyoD1 antibody is available for research use only.
Storage Conditions
Store the MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Image Legend
IHC staining of FFPE human Rhabdomyosarcoma with MyoD1 antibody (clone 5.8A) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P15172

Myoblast determination protein 1

MYOD1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15172
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYOD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O75321
Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

DifferentiationMyogenesisTranscriptionTranscription regulation

Disease

Disease variant

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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