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Podoplanin Antibody

CAT: 0800-V5650-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V5650-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Podoplanin is a transmembrane mucoprotein (38 kDa) recognized by the D2-40 monoclonal antibody. Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin is co-localized with VEGFR3/FLT4, another marker for lymphatic endothelial cells. Podoplanin is selectively expressed in lymphatic endothelium as well as Lymphangiomas, Kaposi’s Sarcomas and in subset Angiosarcomas with probable lymphatic differentiation. Podoplanin has also been shown to be expressed in Epithelioid Mesotheliomas, Hemangioblastomas and Seminomas.
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q86YL7
Host
Mouse
Reactivity
Human
Immunogen
Resected tissue from dysgeminoma of the ovary was used as the immunogen for the Podoplanin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
D2-40
Applications
IHC-P
Purity
Protein G affinity
Format
Purified
Limitations
This Podoplanin antibody is available for research use only.
Storage Conditions
Aliquot the Podoplanin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Podoplanin antibody should be determined by the researcher.
Location
Cytoplasm, Membrane
Image Legend
IHC staining of FFPE human tonsil tissue with Podoplanin antibody (clone D2-40) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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