Clusterin Antibody / CLU / APO-J

• Description:

  Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, 伪 and 尾, are associated through disulfide bonds. The 尾 subunit (also called ApoJ伪) corresponds to residues 23-227. The 伪 subunit (also called ApoJ尾) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences 尾-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P10909

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 150-300 from human CLU protein was used as the immunogen for the Clusterin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CLU/12058

• Applications:

  IHC-P

• Purity:

  Protein G affinity

• Format:

  Purified

• Limitations:

  This Clusterin antibody is available for research use only.

• Storage Conditions:

  Aliquot the Clusterin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Clusterin antibody should be determined by the researcher.

• Location:

  Cytoplasm, Secreted

• Image Legend:

  IHC staining of FFPE human adrenal gland tissue with Clusterin antibody (clone CLU/12058) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.