HLA-E Antibody / MHC class I antigen E

CAT: 0800-V5733-20UGSize: 20 µgDry Ice: NoHazardous: No

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CAT#:0800-V5733-20UGSize:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs) . MHC class I molecules consist of two polypeptide chains, an a or heavy chain and a non-covalently associated protein, b-2-Microglobulin. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A is a MHC class I heavy chain molecule that plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and HLA-C are proteins encoded by closely related genes that also exist in the MHC class I. HLA-E belongs to the HLA class I heavy chain paralogs. HLA-E is a heterodimer consisting of a heavy chain and a light chain. The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P13747

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-150 from human HLAE protein was used as the immunogen for the HLA-E antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

HLAE/9468

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This HLA-E antibody is available for research use only.

Storage Conditions

Aliquot the HLA-E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HLA-E antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human tonsil tissue with HLA-E antibody (clone HLAE/9468) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P13747

HLA class I histocompatibility antigen, alpha chain E

HLAE_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P13747
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: HLA-E
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: E2G051, Q30169, Q6DU44, Q9BT83, Q9GIY7, Q9GIY8

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityHost-virus interactionImmunityInnate immunity

Cellular component

Cell membraneGolgi apparatusMembraneMHC ISecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
H6098-68	HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E)
GTR18716915	HLA-E Antibody / MHC class I antigen E
046240-APC	HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E) (APC)
046240-PE	HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E) (PE)
H6098-68F	HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E, HLA-6.2, HLAE)
H6098-68G	HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E, HLA-6.2, HLAE) discontinued
H6098-68B	HLA Class 1 Antigen E (HLA Class I Histocompatibility Antigen alpha Chain E, HLAE, HLA-E, HLA-6.2, EA1.2, EA2.1, Major Histocompatibility Complex Class I E, MHC Class I Antigen E, QA1)
482786	HLA-E (HLA Class I Histocompatibility Antigen, Alpha Chain E, Major Histocompatibility Complex, Class I, E)
127893-Biotin	HLA Class 1 Antigen E (HLA Class I Histocompatibility Antigen alpha Chain E, HLAE, HLA-E, HLA-6.2, EA1.2, EA2.1, Major Histocompatibility Complex Class I E, MHC Class I Antigen E, QA1) (Biotin)
MBS6480923-01	HLA Class 1 Antigen E (HLA Class I Histocompatibility Antigen alpha Chain E, HLAE, HLA-E, HLA-6.2, EA1.2, EA2.1, Major Histocompatibility Complex Class I E, MHC Class I Antigen E, QA1) (FITC)