Recombinant Ubiquitin Antibody

• Description:

  Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P62979, P62987

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein from human Ubiquitin protein (within amino acids 1-119) was used as the immunogen for the recombinant Ubiquitin antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  RUBB/9476

• Applications:

  IHC-P, WB

• Purity:

  Protein G affinity

• Format:

  Purified

• Limitations:

  This recombinant Ubiquitin antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant Ubiquitin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the recombinant Ubiquitin antibody should be determined by the researcher.

• Location:

  Cell membrane, cytoplasmic, nuclear

• Image Legend:

  IHC staining of FFPE human brain tissue with recombinant Ubiquitin antibody (clone rUBB/9476) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.