Anti-Olig2 Antibody

• Background:

  Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)

• Description:

  Our Anti-Olig2 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Olig2 and is protein a purified. It is great for use in WB, IHC, ICC, IP.

• Synonyms:

  Basic domain helix loop helix protein class B 1 antibody, Basic helix loop helix protein class B 1 antibody, BHLHB antibody, bHLHB1 antibody, bHLHe19 antibody, Class B basic helix loop helix protein 1 antibody, Class B basic helix-loop-helix protein 1 antibody, class E basic helix loop helix protein 19 antibody, Class E basic helix-loop-helix protein 19 antibody, Human protein kinase C binding protein RACK17 antibody, Olig2 antibody, OLIG2_HUMAN antibody, Oligo2 antibody, Oligodendrocyte lineage transcription factor 2 antibody, Oligodendrocyte specific bHLH transcription factor 2 antibody, Oligodendrocyte transcription factor 2 antibody, OTTHUMP00000067569 antibody, OTTHUMP00000067570 antibody, PRKCBP2 antibody, Protein kinase C binding protein 2 antibody, Protein kinase C binding protein RACK17 antibody, Protein kinase C-binding protein 2 antibody, Protein kinase C-binding protein RACK17 antibody, RACK17 antibody

• Other Statements:

  For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

• Gene Name:

  OLIG2

• NCBI Gene ID:

  50913

• UniProt:

  Q9EQW6

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, and Rat

• Immunogen:

  Recombinant mouse Olig2.

• Immunogen Type:

  Mouse

• Target:

  Olig2

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, IHC, ICC, IP

• Concentration:

  0.5 mg/ml

• Format:

  Protein A purified

• Form:

  Liquid

• Buffer:

  100 mM Glycine, 200 mM Tris, pH 8.0

• Molecular Weight:

  27

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

• IHC Dilution:

  1:200-1:1000

• WB Dilution:

  1:3000

• IP Dilution:

  1:500-1:1000

• Specificity:

  Specific for endogenous levels of the ~32 kDa Olig2 protein.

• Formulation:

  Protein A purified rabbit serum

• ICC Dilution:

  1:1000

• Quality Control:

  Western blots performed on each lot.

• Isotype:

  IgG