Anti-Nuclei Antibody

• Background:

  This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

• Description:

  Our Anti-Nuclei primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in IHC, ICC.

• Synonyms:

  HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

• Other Statements:

  For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

• Gene Name:

  Marker for human cells in xenographic research

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human cell homogenate.

• Immunogen Type:

  Human

• Target:

  Nuclei

• Clonality:

  Monoclonal

• Clone:

  235-1

• Conjugation:

  Unconjugated

• Applications:

  ELISA, IHC, ICC

• Concentration:

  1 mg/ml

• Format:

  Concentrated tissue culture supernatant

• Form:

  Liquid

• Buffer:

  Concentrated tissue culture supernatent

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

• IHC Dilution:

  1:100

• Specificity:

  Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

• Formulation:

  Concentrated tissue ulture supernatant

• ICC Dilution:

  1:100

• Quality Control:

  Immunofluorescence performed on each lot.

• Isotype:

  IgG1