Anti-eIF4A2 Antibody

• Background:

  The eIF4A, eukaryotic initiation factor 4A, are a set of proteins, eIF4A1, eIF4A2, and eIF4A3, that belong to the extensive DEAD-box RNA helicase family (Weinstein et al., 1997) . They are involved in many aspects of RNA metabolism by virtue of their RNA-binding capacity and ATPase activity (Lu et al., 2014) . eIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome (Gingras et al., 1999) . In humans eIF4A2 is expressed in all normal tissues, though highly expressed in skeletal muscle (Sudo et al., 1995) . Studies suggest eIF4A2 may play a role in some cancers, specifically breast and lung cancer, along with melanoma (Shaoyan et al., 2013) .

• Description:

  Our Anti-eIF4A2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eIF4A2 and is antigen affinity purified from pooled serum. It is great for use in WB.

• Synonyms:

  ATP dependent RNA helicase eIF4A 2 antibody, ATP-dependent RNA helicase eIF4A-2 antibody, BM-010 antibody, DDX2B antibody, eIF 4A II antibody, eIF-4A-II antibody, EIF4A antibody, eIF4A II antibody, eIF4A-II antibody, EIF4A2 antibody, EIF4F antibody, Eukaryotic initiation factor 4A II antibody, Eukaryotic initiation factor 4A-II antibody, eukaryotic translation initiation factor 4A isoform 2 antibody, Eukaryotic translation initiation factor 4A2 antibody, IF4A2_HUMAN antibody, N-terminally processed antibody

• Other Statements:

  For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

• Gene Name:

  EIF4A2

• NCBI Gene ID:

  1917

• UniProt:

  Q14240

• Host:

  Rabbit

• Reactivity:

  Human and Mouse

• Immunogen:

  Synthetic peptide from the N-terminal region of the human eIF4A2 protein.

• Immunogen Type:

  Human

• Target:

  EIF4A2

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB

• Concentration:

  Lot Dependent

• Format:

  Antigen Affinity Purified from Pooled Serum

• Form:

  Liquid

• Buffer:

  10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

• Molecular Weight:

  46

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• WB Dilution:

  1:1000

• Specificity:

  Specific for endogenous levels of the ~46 kDa eIF4A2 protein.

• Formulation:

  Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.

• Quality Control:

  Western blots performed on each lot.

• Species:

  Bovine, Non-Human Primate, Rat

• Isotype:

  IgG