Anti-ATF2 (Thr52) Antibody

• Background:

  The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008) . Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008) . To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr-52 by PKCε regulates this translocation (Lau et al., 2012) .

• Description:

  Our Anti-ATF2 (Thr52) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Thr52) and is antigen affinity purified from pooled serum. It is great for use in WB.

• Synonyms:

  Activating transcription factor 2 antibody, Activating transcription factor 2 splice variant ATF2 var2 antibody, ATF 2 antibody, Atf-2 antibody, Atf2 antibody, ATF2 protein antibody, ATF2_HUMAN antibody, cAMP Response Element Binding Protein 2 antibody, cAMP response element binding protein CRE BP1 antibody, cAMP response element-binding protein CRE-BP1 antibody, cAMP responsive element binding protein 2, formerly antibody, cAMP-dependent transcription factor ATF-2 antibody, cAMP-responsive element-binding protein 2 antibody, CRE BP1 antibody, CRE-BP antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, CREBP1 antibody, Cyclic AMP dependent transcription factor ATF 2 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, D130078H02Rik antibody, D18875 antibody, HB 16 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, MGC105211 antibody, MGC105222 antibody, MGC111558 antibody, MGC142504 antibody, mXBP antibody, MXBP protein antibody, Tg (Gzma-Klra1) 7Wum antibody, TREB 7 antibody, TREB7 antibody

• Other Statements:

  For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

• Gene Name:

  ATF2

• NCBI Gene ID:

  1386

• UniProt:

  P15336

• Modification Site:

  Thr52

• Host:

  Rabbit

• Reactivity:

  Human and Mouse

• Immunogen:

  Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH) .

• Immunogen Type:

  Human

• Target:

  ATF2 (Thr52)

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB

• Concentration:

  Lot Dependent

• Format:

  Antigen Affinity Purified from Pooled Serum

• Form:

  Liquid

• Buffer:

  10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

• Modification:

  Phosphorylated

• Molecular Weight:

  74

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• WB Dilution:

  1:250

• Specificity:

  Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.

• Formulation:

  Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

• Quality Control:

  Western blots performed on each lot.

• Isotype:

  IgG