10X Lysis Buffer

• Background:

  Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.

• Description:

  Our 1% SDS lysis buffer from PhosphoSolutions is made in-house. It fully solubilizes membrane and other hard-to-solubilize proteins for WB. Suitable for both organs and tissue culture cells.

• Other Statements:

  This is a research laboratory reagent and is not for use as diagnostic or therapeutic reagents in humans.

• Concentration:

  10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0.

• Format:

  SDS Lysis Buffer

• Form:

  Liquid

• Buffer:

  10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0. Dilute to 1X with dH2O before use.

• Shipping Conditions:

  Ambient

• Storage Conditions:

  Storage at room temperature is recommended for both 10X and 1X solutions to prevent SDS from precipitating out of solution.