Anti-Parkin Antibody

• Background:

  Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

• Description:

  Our Parkin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Parkin and is antigen affinity purified. It is great for use in WB.

• Synonyms:

  PRKN, E3 ubiquitin-protein ligase parkin, PARK2

• Other Statements:

  For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

• Gene Name:

  PRKN

• UniProt:

  O60260

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide corresponding to amino acids from the N-terminal region of human Parkin protein.

• Immunogen Type:

  Human

• Target:

  Parkin

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB

• Format:

  Antigen Affinity Purified

• Form:

  Liquid

• Buffer:

  PBS + 0.02% NaN3

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

• WB Dilution:

  1:500-1:1000

• Specificity:

  Reacts with the N-terminal region of the 52 kDa human Parkin protein.

• Formulation:

  Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

• Quality Control:

  Western blots performed on each lot.

• Isotype:

  IgG