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Beta2-Microglobulin Antibody

CAT: 0013-GTR17856814Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR17856814Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Anti-Hu beta2-Microglobulin Purified
Product Name Alternative
B2M
UniProt
P61769
Reactivity
Human
Immunogen
Purified human beta2-microglobulin
Target
Beta2-Microglobulin
Clonality
Monoclonal
Clone
B2M-01
Conjugation
Unconjugated
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
Tested Applications
ELISA, FC, ICC, IHC-P, IP, RIA, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG2a
Entrez
567

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