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Human C4 (Complement Component 4) ELISA Kit

CAT: 1444-EH26425Size: 96 TestsDry Ice: NoHazardous: No
CAT#:1444-EH26425Size:96 Tests
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Synonyms
C4
Cross Reactivity
The kit detected Human C4 in the samples and no significant cross-species reactions were found
Target
C4
Type
Colormetric
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4. You can calculate the concentration of Human C4 in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000ng/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
6.4ng/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

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