Human MHCE/HLA-E (Major Histocompatibility Complex Class I E) ELISA Kit

• Synonyms:

  HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E

• Cross Reactivity:

  The kit detected Human MHCE/HLA-E in the samples and no significant cross-species reactions were found

• Target:

  MHCE/HLA-E

• Type:

  Colormetric

• Detection Method:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MHCE/HLA-E. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MHCE/HLA-E and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MHCE/HLA-E, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MHCE/HLA-E. You can calculate the concentration of Human MHCE/HLA-E in the samples by comparing the OD of the samples to the standard curve.

• Assay Type:

  Sandwich

• Assay Performance Time:

  3.25h

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  6.25-400pg/mL

• Precision:

  CV<10%

• Recovery:

  80%-120%

• Sensitivity:

  2.27pg/mL

• Storage Conditions:

  Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

• Species:

  Human