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Human CPP (Copeptin) ELISA Kit

CAT: 1444-EH20050Size: 96 TestsDry Ice: NoHazardous: No
CAT#:1444-EH20050Size:96 Tests
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Synonyms
CT-proAVP; C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
UniProt
P01185 (126~164aa)
Cross Reactivity
The kit detected Human CPP in the samples and no significant cross-species reactions were found
Target
CPP
Type
Colormetric
Field of Research
Neuroscience, Signal transduction
Detection Method
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human CPP. During the reaction, Human CPP in the sample or standard competes with a fixed amount of Human CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Type
Competitive
Assay Performance Time
1.5h
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
18.75pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

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