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Mouse MBP (Myelin Basic Protein) ELISA Kit

CAT: 1444-EM10555Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EM10555Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
Myelin_MBP
UniProt
P04370
Cross Reactivity
The kit detected Mouse MBP in the samples and no significant cross-species reactions were found
Target
MBP
Type
Colormetric
Field of Research
Cancer, Neuroscience, Tags and Cell Markers
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MBP. You can calculate the concentration of Mouse MBP in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10ng/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
0.1ng/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Mouse

UniProtKB · P04370

Myelin basic protein

MBP_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P04370
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mbp
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
MBP
EC number
—
Processing
—
Secondary accessions
Q01585, Q03139, Q03176, Q61836, Q61837, Q99KE4, Q9QWP1
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationCitrullinationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Autoimmune encephalomyelitis

Cellular component

Cell membraneCytoplasmMembraneNucleus

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