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Human Irisin (Irisin) ELISA Kit

CAT: 1444-EH10049Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EH10049Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
Irisin
UniProt
Q8NAU1
Cross Reactivity
The kit detected Human Irisin in the samples and no significant cross-species reactions were found
Target
Irisin
Type
Colormetric
Field of Research
Cell Biology, Metabolism
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Irisin. You can calculate the concentration of Human Irisin in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
9.38pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

UniProtKB · Q8NAU1

Fibronectin type III domain-containing protein 5

FNDC5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8NAU1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FNDC5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A0AA34QVI5, A6NMC9, D3DPQ6, Q6P6D9, Q7Z676
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative promoter usageAlternative splicing

Cellular component

Cell membraneMembranePeroxisomeSecreted

PTM

Glycoprotein

Molecular function

Hormone

Domain

TransmembraneTransmembrane helix

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